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Issue 24, 2018
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FRET and PET paired dual mechanistic carbon dots approach for tyrosinase sensing

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Abstract

A dual mechanistic FRET and PET paired ratiometric fluorescence sensor probe has been prepared using carbon dots and naphthalimide fluorophores. The carbon dots are covalently joined with a naphthalimide moiety to develop the FRET phenomenon, which emits at two different wavelengths (i.e., λmax = 440 and 540 nm). However, on catalytic reaction of tyrosinase, the fluorescence emission intensity of the acceptor unit at 540 nm is quenched gradually, owing to the switching on of the PET mechanism; while emission of the donor unit remains significantly unaffected. The probe exhibits high selectivity and specificity towards tyrosinase in complex biological medium with a detection limit of 1.2 U mL−1. Moreover, endogenous images of tyrosinase in B16 cells have been observed under a confocal laser-scanning microscope.

Graphical abstract: FRET and PET paired dual mechanistic carbon dots approach for tyrosinase sensing

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Supplementary files

Article information


Submitted
23 Feb 2018
Accepted
18 May 2018
First published
21 May 2018

J. Mater. Chem. B, 2018,6, 4139-4145
Article type
Paper

FRET and PET paired dual mechanistic carbon dots approach for tyrosinase sensing

J. S. Sidhu and N. Singh, J. Mater. Chem. B, 2018, 6, 4139
DOI: 10.1039/C8TB00512E

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