Jump to main content
Jump to site search

Issue 15, 2018
Previous Article Next Article

The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

Author affiliations

Abstract

Nanoparticles offer a promising avenue for the targeted delivery of therapies. To slow clearance, nanoparticles are frequently stealth-coated to prevent opsonization and immune recognition. Serum albumin (SA) has been used as a bio-inspired stealth coating. To develop this shielding strategy for clinical applications, it is critical to understand the interactions between the immune system and SA-camouflaged nanoparticles. This work investigates the in vivo processing of SA-coated nanoparticles using tobacco mosaic virus (TMV) as a model system. In comparing four different SA-formulations, the particles with high SA coverage conjugated to TMV via a short linker performed the best at preventing antibody recognition. All formulations led to similar levels of TMV-specific antibodies after repeat administration in mice; importantly though, SA-specific antibodies were not detected and the TMV-specific antibodies were unable to recognize shielded SA-coated TMV. Upon uptake in macrophages, the shielding agent and nanoparticle separate, where TMV trafficked to the lysosome and SA appears to recycle. The distinct intracellular fates of the TMV carrier and SA shielding agent explain why anti-TMV but not SA-specific antibodies are generated. This work characterizes the outcomes of SA-camouflaged TMV after immune recognition, and highlights the effectiveness of SA as a nanoparticle shielding agent.

Graphical abstract: The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

Back to tab navigation

Article information


Submitted
30 Nov 2017
Accepted
21 Feb 2018
First published
27 Feb 2018

J. Mater. Chem. B, 2018,6, 2204-2216
Article type
Paper

The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

N. M. Gulati, A. S. Pitek, A. E. Czapar, P. L. Stewart and N. F. Steinmetz, J. Mater. Chem. B, 2018, 6, 2204 DOI: 10.1039/C7TB03106H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements