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Issue 14, 2018
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Eu/Tb luminescence for alkaline phosphatase and β-galactosidase assay in hydrogels and on paper devices

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Abstract

Simple technologies for efficient detection of important (bio)molecules are always in great demand. We now report the detection and assay of two biologically important enzymes, alkaline phosphatase and β-galactosidase, in Eu- or Tb-based cholate hydrogels, respectively, and on filter paper discs coated with such hydrogels. Pro-sensitizers derived from 1-hydroxypyrene and 2,3-dihydroxynaphthalene were incorporated into Eu or Tb cholate hydrogels, respectively. Upon enzyme action, these artificial substrates liberate free sensitizers both in the gel and on gel-coated discs, resulting in turn-on luminescence, red/magenta for Eu, and green for Tb. The detection of enzymes was also demonstrated in natural/biological samples using low-cost systems.

Graphical abstract: Eu/Tb luminescence for alkaline phosphatase and β-galactosidase assay in hydrogels and on paper devices

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Supplementary files

Article information


Submitted
06 Oct 2017
Accepted
06 Mar 2018
First published
06 Mar 2018

J. Mater. Chem. B, 2018,6, 2143-2150
Article type
Paper

Eu/Tb luminescence for alkaline phosphatase and β-galactosidase assay in hydrogels and on paper devices

T. Gorai and U. Maitra, J. Mater. Chem. B, 2018, 6, 2143
DOI: 10.1039/C7TB02657A

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