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Issue 12, 2018
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Bacterial and archaeal community structure involved in biofuels production using hydrothermal- and enzymatic-pretreated sugarcane bagasse for an improvement in hydrogen and methane production

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Abstract

Sugarcane bagasse (SCB) was used as a lignocellulosic substrate, combining the co-production of H2 (Stage I) and CH4 (Stage II) by a dark fermentation process in batch reactors. Hydrothermally- and enzymatic (Aspergillus niger)-pretreated SCB were applied as substrate sources. Two fermentative inocula (In1 and In2) were used in Stage I and a methanogenic inoculum in Stage II (In3), comprising in total three experimental series in relation to Stage I: A (In1), B (In1 plus In2), and C (In2). The final metabolites (solid, liquid, and gaseous fractions) from Stage I were used for CH4 production (Stage II). The SCB pretreatment employed was favorable for biogas and organic acids production. Higher H2 and CH4 yields were obtained in C (4.3 and 6.3 mmol g−1 SCB, respectively). For all conditions, the H2 production occurred primarily via an acetic acid route. The predominance of cellulolytic enzyme producers (Enterococcus and Clostridium) may have favored the H2 and subsequent CH4 production; this last was produced mainly from members of the Methanoregulaceae and Methanosaetaceae families. Furthermore, homoacetogenic bacteria (Acetobacterium, Clostridium, Eubacterium, Holophaga) were also identified in both stages. The synergistic action of these microbial groups promoted the hydrolysis of SCB as well as hydrogen and methane production.

Graphical abstract: Bacterial and archaeal community structure involved in biofuels production using hydrothermal- and enzymatic-pretreated sugarcane bagasse for an improvement in hydrogen and methane production

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Article information


Submitted
29 Jun 2018
Accepted
06 Sep 2018
First published
06 Sep 2018

Sustainable Energy Fuels, 2018,2, 2644-2660
Article type
Paper

Bacterial and archaeal community structure involved in biofuels production using hydrothermal- and enzymatic-pretreated sugarcane bagasse for an improvement in hydrogen and methane production

J. K. Braga, F. Motteran, I. K. Sakamoto and M. B. A. Varesche, Sustainable Energy Fuels, 2018, 2, 2644
DOI: 10.1039/C8SE00312B

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