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Bacterial and archaeal community structure involved in biofuels production using hydrothermal and enzymatic pretreated sugarcane bagasse for hydrogen and methane improvement

Abstract

Sugarcane bagasse (SCB) was used as lignocellulosic substrate, combining the co-production of H2 (stage I) and CH4 (stage II) by dark fermentation process in batch reactors. Hydrothermally and enzymatic (Aspergillus niger) pretreatead SCB were applied as substrate sources. Two fermentative inocula (In1 and In2) were used in stage I and a methanogenic inoculum in stage II (In3), being in total three experimental series in relates to stage I: A (In1), B (In1 plus In2) and C (In2). The final metabolites from stage I were transferred to a second reactor for CH4 production (stage II). The SCB pretreatment employed was favorable for biogas and organic acids production. The higher H2 and CH4 yields were in C (4.3 and 6.3 mmol/g SCB, respectively). For all conditions, the H2 production occurred primarily via acetic acid route. Predominance of cellulolytic enzymes producers (Enterococcus and Clostridium) may have favored the H2 and subsequent CH4 production; this last produced mainly from members of Methanoregulaceae and Methanosaetaceae families. Furthermore, homoacetogenic bacteria (Acetobacterium, Clostridium, Eubacterium, Holophaga) were also identified. The synergistic action of these microbial groups promoted the hydrolysis of SCB and hydrogen and methane production.

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Publication details

The article was received on 29 Jun 2018, accepted on 06 Sep 2018 and first published on 06 Sep 2018


Article type: Paper
DOI: 10.1039/C8SE00312B
Citation: Sustainable Energy Fuels, 2018, Accepted Manuscript
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    Bacterial and archaeal community structure involved in biofuels production using hydrothermal and enzymatic pretreated sugarcane bagasse for hydrogen and methane improvement

    J. K. Braga, F. Motteran, I. K. Sakamoto and M. B. A. Varesche, Sustainable Energy Fuels, 2018, Accepted Manuscript , DOI: 10.1039/C8SE00312B

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