Jump to main content
Jump to site search

Issue 39, 2018
Previous Article Next Article

Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes

Author affiliations

Abstract

Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙ with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙ and NIR-O2˙) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙ in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙ level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙ was also used to elucidate the protective effects of L-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙ in complex nephrotoxicity disease systems.

Graphical abstract: Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Jul 2018, accepted on 10 Sep 2018 and first published on 13 Sep 2018


Article type: Edge Article
DOI: 10.1039/C8SC03308K
Citation: Chem. Sci., 2018,9, 7606-7613
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes

    Y. Lv, Dan Cheng, Dongdong Su, M. Chen, B. Yin, L. Yuan and X. Zhang, Chem. Sci., 2018, 9, 7606
    DOI: 10.1039/C8SC03308K

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements