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Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers

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Abstract

Triblock copolymer of poly(ethylene glycol)-b-poly(2-dimethylaminoethyl methacrylate)-b-poly(pyrenylmethyl methacrylate) (PEG-b-PDMAEMA-b-PPy) has been developed for use as an ideal gene delivery system, which showed both high stability under physiological conditions and efficient gene release in a mimetic cancer environment. The siRNA release from this system without external stimulation was 16% in 1 h and then remained steady. However, the photo-triggered siRNA release was 78% within 1 h and was higher than 91% after 24 h. The remarkable contrast between the stability and release efficiency of these siRNA-condensed micelleplexes before and after photo-irradiation has been rationalized by the light- and pH-induced structural transitions of the triblock copolymer micelles. The negligible cytotoxicity, high cellular uptake efficiency, and remarkable knockdown efficiency shown in in vitro tests further revealed the promising potential of these triblock copolymer micelleplexes for use in stimuli-responsive gene therapy.

Graphical abstract: Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers

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Publication details

The article was received on 02 Apr 2018, accepted on 03 Oct 2018 and first published on 04 Oct 2018


Article type: Edge Article
DOI: 10.1039/C8SC01494A
Citation: Chem. Sci., 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers

    H. Lu, C. Huang, T. Shiue, F. Wang, K. Chang, Y. Chen and C. Peng, Chem. Sci., 2018, Advance Article , DOI: 10.1039/C8SC01494A

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