Issue 10, 2018

Label-free and live cell imaging by interferometric scattering microscopy

Abstract

Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena.

Graphical abstract: Label-free and live cell imaging by interferometric scattering microscopy

Supplementary files

Article information

Article type
Edge Article
Submitted
02 Nov 2017
Accepted
08 Feb 2018
First published
09 Feb 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 2690-2697

Label-free and live cell imaging by interferometric scattering microscopy

J. Park, I. Lee, H. Moon, J. Joo, K. Kim, S. Hong and M. Cho, Chem. Sci., 2018, 9, 2690 DOI: 10.1039/C7SC04733A

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