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Issue 71, 2018
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19F multiple-quantum coherence NMR spectroscopy for probing protein–ligand interactions

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Abstract

A new 19F NMR method is presented which can be used to detect weak protein binding of small molecules with up to mM affinity. The method capitalizes on the synthetic availability of unique SF5 containing compounds and the generation of five-quantum coherences (5QC). Given the high sensitivity of 5QC relaxation to exchange events (i.e. reversible protein binding) fragments which bind to the target with weak affinity can be identified. The utility of the method in early stage drug discovery programs is demonstrated with applications to two model proteins, the neurotoxic NGAL and the prominent tumor target β-catenin.

Graphical abstract: 19F multiple-quantum coherence NMR spectroscopy for probing protein–ligand interactions

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Submitted
10 Nov 2018
Accepted
28 Nov 2018
First published
05 Dec 2018

This article is Open Access

RSC Adv., 2018,8, 40687-40692
Article type
Paper

19 F multiple-quantum coherence NMR spectroscopy for probing protein–ligand interactions

A. Zawadzka-Kazimierczuk, M. Somlyay, H. Kaehlig, G. Iakobson, P. Beier and R. Konrat, RSC Adv., 2018, 8, 40687
DOI: 10.1039/C8RA09296F

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