Jump to main content
Jump to site search

Issue 54, 2018, Issue in Progress
Previous Article Next Article

One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water

Author affiliations

Abstract

Immobilisation of bacteria on or into a polymer support is a common method for the utilisation of bacteria as biocatalysts for many biotechnological, medical and environmental applications. The main challenge in this approach is the time taken for the formation of stable biofilms, and the typically low percentage of bacterial cells present on or in the polymer matrix. In this work we propose a novel method for producing a porous bacteria based structure with the properties of a sponge (bacterial sponge) that we then use as a bioreactor for water treatment. Cryogelation has been used as a tool to create macroporous (i.e. with pores in the range 10–100 μm), highly permeable systems with low diffusion constraints and high bacterial content (more than 98% to total material content). A novel crosslinking system was used to form stable bacterial sponges with a high percentage of live bacteria organized in a 3D porous structure. The bacterial sponge was produced in a one step process and can be made from one or several bacterial strains (in this case, two bacterial strains Pseudomonas mendocina and Rhodoccocus koreensis (and a mixture of both) were used). Reduction of the total polymer content to 2% makes the system more sustainable and environmentally friendly under disposal as it can be simply composted. The bacterial sponges have good mechanical stability and cell viability, which enables repeated use of the materials for phenol degradation for up to five weeks. The material can be stored and transported in cryogenic conditions (−80 °C) for prolonged periods of time, retaining its bioremediation activity following 4–6 weeks of frozen storage. The proposed method of producing bioreactors with a high number of live immobilised bacteria, low polymer content and controlled 3D structure is a promising tool for developing novel materials based on active bacterial cells for various environmental, biotechnological, biological and medical applications.

Graphical abstract: One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water

Back to tab navigation

Supplementary files

Publication details

The article was received on 17 May 2018, accepted on 14 Aug 2018 and first published on 03 Sep 2018


Article type: Paper
DOI: 10.1039/C8RA04219E
RSC Adv., 2018,8, 30813-30824
  • Open access: Creative Commons BY license
  •   Request permissions

    One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water

    A. K. Al-Jwaid, D. Berillo, I. N. Savina, A. B. Cundy and J. L. Caplin, RSC Adv., 2018, 8, 30813
    DOI: 10.1039/C8RA04219E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements