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Issue 20, 2018
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Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

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Abstract

Quercetin spectrofluorometric quantification was carried out in aqueous media (pH 7) using micelles of surfactants, namely: CTAB, SDS and TX100 as promoters of quercetin fluorescence, having a critical micelle concentration, CMC, of: 0.94 ± 0.03, 7.7 ± 0.6 and 0.18 ± 0.3 mM, respectively, measured through UV-Vis spectrophotometry. The thermodynamic binding constant, K, for the quercetin-surfactants' micelles supramolecular complex was estimated to be (log(K/M−1)) = 2.87 ± 0.02, 2.78 ± 0.04 and 2.80 ± 0.04, respectively, from fluorescence spectrophotometry. With the aid of these parameters it was possible to construct species distribution diagrams and choose the experimental conditions where quercetin can be quantified in aqueous media from fluorescence measurements. The best lowest limit of detection (0.244 ± 0.092) μM was achieved with CTAB micelles while the best sensitivity (2.919 ± 0.054) M−1 corresponded to SDS.

Graphical abstract: Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

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Supplementary files

Article information


Submitted
07 Feb 2018
Accepted
14 Mar 2018
First published
20 Mar 2018

This article is Open Access

RSC Adv., 2018,8, 10980-10986
Article type
Paper

Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

J. C. Alva-Ensastegui, M. Palomar-Pardavé, M. Romero-Romo and M. T. Ramírez-Silva, RSC Adv., 2018, 8, 10980
DOI: 10.1039/C8RA01213J

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