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Stapling of two PEGylated side chains increases the conformational stability of the WW domain via an entropic effect

Abstract

Hydrocarbon stapling and PEGylation are distinct strategies for enhancing the conformational stability and/or pharmacokinetic properties of peptide and protein drugs. Here we combine these approaches by incorporating asparagine-linked O-allyl PEG oligomers at two positions within the β-sheet protein WW, followed by stapling of the PEGs via olefin metathesis. The impact of stapling two sites that are close in primary sequence is small relative to the impact of PEGylation alone and depends strongly on PEG length. In contrast, stapling of two PEGs that are far apart in primary sequence but close in tertiary structure pro-vides substantially more stabilization, derived mostly from an entropic effect. Comparison of PEGylation + stapling vs. alkylation + stapling at the same positions in WW reveals that both approaches provide simi-lar overall levels of conformational stability.

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Publication details

The article was received on 12 Oct 2018, accepted on 08 Nov 2018 and first published on 09 Nov 2018


Article type: Communication
DOI: 10.1039/C8OB02535E
Citation: Org. Biomol. Chem., 2018, Accepted Manuscript
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    Stapling of two PEGylated side chains increases the conformational stability of the WW domain via an entropic effect

    Q. Xiao, N. A. Bécar, N. P. Brown, M. S. Smith, K. L. Stern, S. R. E. Draper, K. P. Thompson and J. L. Price, Org. Biomol. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8OB02535E

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