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Issue 46, 2018
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The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

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Abstract

The study of complex multivalent carbohydrate–protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Using tetra- and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodynamic parameters of multivalent interactions going from the micro to the nanomolar range.

Graphical abstract: The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

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Publication details

The article was received on 13 Jul 2018, accepted on 17 Aug 2018 and first published on 28 Sep 2018


Article type: Communication
DOI: 10.1039/C8OB01664J
Citation: Org. Biomol. Chem., 2018,16, 8899-8903
  • Open access: Creative Commons BY-NC license
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    The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

    E. Laigre, D. Goyard, C. Tiertant, J. Dejeu and O. Renaudet, Org. Biomol. Chem., 2018, 16, 8899
    DOI: 10.1039/C8OB01664J

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