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Issue 22, 2018
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Energy transfer chemiluminescence for ratiometric pH imaging

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Chemiluminescence imaging offers a low background and high sensitivity approach to imaging analytes in living cells and animals. Intensity-based measurements have been developed, but require careful consideration of kinetics, probe localization, and fluctuations in quantum yield, all of which complicate quantification. Here, we report a ratiometric strategy for quantitative chemiluminescence imaging of pH. The strategy relies on an energy transfer cascade of chemiluminescence emission from a spiroadamantane 1,2-dioxetane to a ratiometric pH indicator via fluorescent dyes in Enhancer solutions. Monitoring the pH-dependent changes in chemiluminescence emission at multiple wavelengths enables ratiometric imaging and quantification of pH independent from variations due to kinetics and probe concentration.

Graphical abstract: Energy transfer chemiluminescence for ratiometric pH imaging

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The article was received on 25 Apr 2018, accepted on 17 May 2018 and first published on 17 May 2018

Article type: Paper
DOI: 10.1039/C8OB00972D
Citation: Org. Biomol. Chem., 2018,16, 4176-4182
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    Energy transfer chemiluminescence for ratiometric pH imaging

    W. An, R. P. Mason and A. R. Lippert, Org. Biomol. Chem., 2018, 16, 4176
    DOI: 10.1039/C8OB00972D

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