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Tailoring Pore Structure with Optimal Mesopores to Remarkably Promote DNA Adsorption Guiding Growth of Active Mn3(PO4)2 toward Sensitive Superoxide Biomimetic Enzyme Sensor

Abstract

The great challenge to fabricate a biomimetic enzyme sensor is to have sensitivity and selectivity equal to or better than its corresponding biological one. Porous electrodes possess large surface area and are often used to greatly improve the sensor sensitivity. However, how to tailor the pore structure, especially the pore sizes distribution to further improve the sensitivity and selectivity of a biomimetic sensor has not been investigated yet. Superoxide anion (O2•−) plays essential roles in various biological processes and is of importance in clinical diagnosis and life science research. It is generally detected by superoxide dismutase enzyme. Herein, we delicately tailor the pore structure of carbon nanofibers (CNFs) by pyrolysis to have optimal mesopore structure for strong adsorption of DNA, followed by guiding growth of Mn3(PO4)2 as a biomimetic enzyme toward highly sensitive detection of O2•−. The Mn3(PO4)2-DNA/CNFs sensor achieves the best sensitivity among the reported Mn3(PO4)2-based O2•− biomimetic sensors while possessing good selectivity. The enhancement mechanism is also investigated, indicating that the mesopores ratio of CNFs plays an essential role in the high sensitivity and selectivity due to its strong absorption of DNA for guiding growth of a large amount of uniform sensing components, Mn3(PO4)2 toward a high sensitivity and selectivity. The biomimetic sensor was further used to in situ monitor O2•− released from the human keratinocyte cells and human malignant melanoma cells under drug stimulation, showing high sensitivity and good selectivity to real time quantitatively detect O2•−. This work provides a highly sensitive in situ real time biomimetic O2•− sensor for applications in biological research and diagnosis, while shedding a scientific light on the enhancement mechanism of the pore structure, especially the poresize distribution of a porous electrode for high performance sensing process.

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Publication details

The article was received on 02 Nov 2018, accepted on 21 Dec 2018 and first published on 27 Dec 2018


Article type: Communication
DOI: 10.1039/C8NR08829B
Citation: Nanoscale, 2018, Accepted Manuscript
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    Tailoring Pore Structure with Optimal Mesopores to Remarkably Promote DNA Adsorption Guiding Growth of Active Mn3(PO4)2 toward Sensitive Superoxide Biomimetic Enzyme Sensor

    Z. Zou, X. Ma, L. Zou, Z. Z. Shi, Q. Q. Sun, Q. Liu, T. T. Liang and C. M. Li, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR08829B

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