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Issue 40, 2018
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Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy

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Abstract

Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.

Graphical abstract: Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy

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Publication details

The article was received on 13 Sep 2018, accepted on 30 Sep 2018 and first published on 01 Oct 2018


Article type: Paper
DOI: 10.1039/C8NR07481J
Citation: Nanoscale, 2018,10, 19125-19130

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    Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy

    G. L. C. Spicer, L. Almassalha, I. A. Martinez, R. Ellis, J. E. Chandler, S. Gladstein, D. Zhang, T. Nguyen, S. Feder, H. Subramanian, R. de la Rica, S. A. Thompson and V. Backman, Nanoscale, 2018, 10, 19125
    DOI: 10.1039/C8NR07481J

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