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DNA flower-encapsulated horseradish peroxidase with enhanced biocatalytic activity synthesized by an isothermal one-pot method based on rolling circle amplification

Abstract

DNA nanotechnology has been developed to construct a variety of functional two- and three-dimensional structures for versatile applications. Rolling circle amplification (RCA) has become prominent in the assembly of DNA-inorganic composites with hierarchical structures and attractive properties. Here, we demonstrate a one-pot method to directly encapsulate horseradish peroxidase (HRP) in DNA flowers (DFs) during RCA. The growing DNA strands and Mg2PPi crystals lead to the construction of porous DFs, which provide sufficient interaction sites for spontaneously incorporating HRP molecules in DFs with high loading capacity and good stability. Further, in comparison with free HRP, the DNA flowers-encapsulated HRP (termed HRP-DFs) demonstrate enhanced enzymatic activity, which can efficiently biocatalyze the H2O2-mediated etching of gold nanorods (AuNRs) to generate distinct color changes since the longitudinal localized surface plasmon resonance (LSPR) frequency of AuNRs is highly sensitive to the changes in AuNR aspect ratio. Through rationally incorporating complementary thrombin aptamer sequence in the circular template, the synthesized HRP-DFs composites are readily used as amplified labels for visual and colorimetric detection of thrombin with ultrahigh sensitivity and excellent selectivity. Therefore, our proposed strategy for direct encapsulation of enzyme molecules into DNA structures shows considerable potential applications in biosensing, biocatalysis, and point-of-care diagnostics.

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Publication details

The article was received on 26 Sep 2018, accepted on 31 Oct 2018 and first published on 06 Nov 2018


Article type: Paper
DOI: 10.1039/C8NR07294A
Citation: Nanoscale, 2018, Accepted Manuscript
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    DNA flower-encapsulated horseradish peroxidase with enhanced biocatalytic activity synthesized by an isothermal one-pot method based on rolling circle amplification

    Y. Yan, J. Li, W. Li, Y. Wang, W. Song and S. Bi, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR07294A

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