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One-scan fluorescence emission difference nanoscopy developed with excitation orthogonalized upconversion nanoparticles

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Abstract

We experimentally realized one-scan fluorescence emission difference nanoscopy (FED) by simultaneously imaging two different color emissions of NaYF4:Er3+@NaYF4@NaYF4:Yb3+/Tm3+ upconversion nanoparticles. Under the irradiation of two synchronized laser beams, a solid 940 nm beam and a hollow 808 nm beam, green emission of Er3+ and blue emission of Tm3+ can be orthogonally generated and collected. After simple subtraction, a resulting super-resolution image featuring 54 nm resolution was obtained. This strategy of excitation orthogonality would greatly improve the imaging speed and the applicability of FED nanoscopy.

Graphical abstract: One-scan fluorescence emission difference nanoscopy developed with excitation orthogonalized upconversion nanoparticles

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Publication details

The article was received on 29 Aug 2018, accepted on 24 Oct 2018 and first published on 25 Oct 2018


Article type: Communication
DOI: 10.1039/C8NR07017B
Citation: Nanoscale, 2018, Advance Article
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    One-scan fluorescence emission difference nanoscopy developed with excitation orthogonalized upconversion nanoparticles

    B. Huang, Q. Wu, X. Peng, L. Yao, D. Peng and Q. Zhan, Nanoscale, 2018, Advance Article , DOI: 10.1039/C8NR07017B

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