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Chemical pre-reduction and electro-reduction guided preparation of porous graphene bionanocomposite for indole-3-acetic acid detection

Abstract

A porous graphene (PG) bionanocomposites of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposited on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopic (SEM), transmission electron microscopy (TEM) Raman, Energy dispersive spectrometer (EDS), Fourier transform infrared spectra (FTIR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2×10-11 to 2×10-8 g/mL with a detection limit of 0.016 ng/mL (S/N=3) and showed significant linearity R2=0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranged from -5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite film for wide applications.

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Publication details

The article was received on 27 Aug 2018, accepted on 29 Nov 2018 and first published on 29 Nov 2018


Article type: Paper
DOI: 10.1039/C8NR06913A
Citation: Nanoscale, 2018, Accepted Manuscript
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    Chemical pre-reduction and electro-reduction guided preparation of porous graphene bionanocomposite for indole-3-acetic acid detection

    Z. Su, X. Xu, Y. Cheng, Y. Tan, L. Xiao, D. Tang, H. Jiang, X. Qin and H. Wang, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR06913A

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