Jump to main content
Jump to site search


Specific Binding and Internalization: An Investigation of Fluorescent Aptamer-Gold Nanoclusters and Cells with Fluorescence Lifetime Imaging Microscopy

Abstract

Fluorescent gold nanoclusters show promising properties for biological applications. We biofunctionalized fluorescent 11-mercaptoundecanoic-acid stabilized gold nanoclusters (AuNCs) with an aptamer to target the Interleukin-6-receptor expressed on BaF3 cells specifically. Although the fluorescence emission of the AuNCs (535 nm) is in the same wavelength region as the autofluorescence of the cell, we are able to distinguish between nanoclusters and cell using the fluorescence decay time, which is much longer for the AuNCs (100 ns) than for the autofluorescence. After a first short incubation period we detect AuNCs specifically bound to the cell's membrane by using two fluorescence lifetime imaging microscopy (FLIM) methods: gated and direct FLIM. After a second incubation period the previously bound AuNCs are internalized by the cell, as could be resolved solely by the direct FLIM. This proves the superior sensitivity of this method compared to gated FILM. We find that the optical properties of AuNCs do not change upon binding to the cell, but exhibit a change when internalized into the cell, induced by an interaction between AuNCs and cell.

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Aug 2018, accepted on 06 Oct 2018 and first published on 08 Oct 2018


Article type: Paper
DOI: 10.1039/C8NR06639F
Citation: Nanoscale, 2018, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Specific Binding and Internalization: An Investigation of Fluorescent Aptamer-Gold Nanoclusters and Cells with Fluorescence Lifetime Imaging Microscopy

    M. Mutas, C. Strelow, T. Kipp and A. Mews, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR06639F

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements