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Fluorescence immunoassay based on the enzyme cleaving ss-DNA to regulate the synthesis of histone-ds-poly(AT) templated copper nanoparticles

Abstract

Herein, for the first time we reported a novel competitive fluorescence immunoassay for the ultrasensitive detection of aflatoxin B1 (AFB1) using histone-ds-poly(AT) templated copper nanoparticles (His-pAT CuNPs) as the fluorescent indicator. In this immunoassay, glucose oxidase (Gox) was used as the carrier of competing antigen to catalyze the formation of hydrogen peroxide (H2O2) from glucose. H2O2 was converted into hydroxyl radical by Fenton’s reagent, which further regulated the fluorescence signals of His-pAT CuNPs. Owing to the ultrahigh sensitivity of ss-DNA to hydroxyl radical, the proposed fluorescence immunoassay exhibited a favorable dynamic linear detection of AFB1 ranging from 0.46 pg/mL to 400 pg/mL with an extremely half maximal inhibitory concentration and limit of detection at 6.13 and 0.15 pg/mL, respectively. The intra- and inter-assay showed that the average recoveries for AFB1 spiked corn samples ranged from 96.87% to 100.73% and 96.67% to 114.92%, respectively. The reliability of this method was further confirmed by ultra-performance liquid chromatography coupled with fluorescence detector method. In summary, this work offers a novel screening strategy with high sensitivity and robustness for the quantitative detection of mycotoxins or other pollutants in food safety and clinical diagnosis.

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Publication details

The article was received on 01 Aug 2018, accepted on 27 Sep 2018 and first published on 28 Sep 2018


Article type: Paper
DOI: 10.1039/C8NR06175K
Citation: Nanoscale, 2018, Accepted Manuscript
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    Fluorescence immunoassay based on the enzyme cleaving ss-DNA to regulate the synthesis of histone-ds-poly(AT) templated copper nanoparticles

    Y. Xiong, B. Gao, K. Wu, Y. Wu, Y. Chai, X. Huang and Y. Xiong, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR06175K

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