Jump to main content
Jump to site search


Functionalized gold and persistent luminescence nanoparticles based ratiometric absorption and TR-FRET nanoplatform for high-throughput sequential detection of L-cysteine and insulin

Abstract

In vitro diagnostic (IVD) is a crucial component of healthcare systems for early diagnosis of diseases and follow-up therapy, generally makes clinical diagnosis faster, easier, and painless to patients. Herein, we report a dual-signaling nanoplatform for ratiometric absorption and time resolved fluorescence resonance energy transfer (TR-FRET) based on L-cysteine mediated agminated gold nanoparticles (AuNPs) and long afterglow nature of persistent luminescence nanoparticles (PLNPs). With this nanoplatform, we achieved high-throughput sequential detection of L-cysteine and Insulin in human serum without matrix interference. The proposed nanoplatform showed excellent linearity and precision for the determination of L-cysteine in the range of 10 nM to 5.5 μM with a detection limit (LOD) of 2.2 nM, and for the detection of insulin in the range of 12 pM to 3.44 nM with a LOD of 2.06 pM. The developed dual-signaling nanplatform was successfully applied for sequential determination of L-cysteine and insulin in human serum.

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 May 2018, accepted on 10 Jul 2018 and first published on 13 Jul 2018


Article type: Paper
DOI: 10.1039/C8NR04414G
Citation: Nanoscale, 2018, Accepted Manuscript
  •   Request permissions

    Functionalized gold and persistent luminescence nanoparticles based ratiometric absorption and TR-FRET nanoplatform for high-throughput sequential detection of L-cysteine and insulin

    J. Li, Y. cheng, W. Wang and X. Yan, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR04414G

Search articles by author

Spotlight

Advertisements