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Issue 29, 2018
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A graphene-based fluorescent nanoprobe for simultaneous monitoring of miRNA and mRNA in living cells

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Abstract

Since microRNA-21 (miR-21) and protein programmed cell death 4 (PDCD4) are implicated in tumor progression, simultaneous monitoring of the dynamic variation of miR-21 and PDCD4 mRNA in living cells is of great interest for understanding their relationship in the pathology of the disease. Herein, a versatile nanoprobe based on graphene oxide (GO) and DNA was prepared which can simultaneously monitor and visualize miR-21 and PDCD4 mRNA in living cells. In vitro experiments demonstrate that the nanoprobe exhibits outstanding selectivity and high sensitivity towards miR-21 and PDCD4 mRNA. Moreover, by exploiting the DNA/GO sensing platform, simultaneous imaging of miR-21 and PDCD4 mRNA in MCF-7 human breast cancer cells has been realized. More significantly, an inverse correlation between miR-21 and PDCD4 mRNA was verified by fluorescence confocal imaging and RT-PCR results, indicating the distinguishing ability of the nanoprobe at various expression levels of RNAs. The current strategy provides a promising tool to implement the detection of miR-21 and PDCD4 mRNA in cancer cells and can be expected to be applied for other biomolecular sensing.

Graphical abstract: A graphene-based fluorescent nanoprobe for simultaneous monitoring of miRNA and mRNA in living cells

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Publication details

The article was received on 21 May 2018, accepted on 27 Jun 2018 and first published on 29 Jun 2018


Article type: Paper
DOI: 10.1039/C8NR04106G
Citation: Nanoscale, 2018,10, 14264-14271
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    A graphene-based fluorescent nanoprobe for simultaneous monitoring of miRNA and mRNA in living cells

    W. Pan, B. Liu, X. Gao, Z. Yu, X. Liu, N. Li and B. Tang, Nanoscale, 2018, 10, 14264
    DOI: 10.1039/C8NR04106G

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