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Issue 29, 2018
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A supramolecular host–guest complex for heparin binding and sensing

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Heparin is an anionic polysaccharide widely used in clinics as an anticoagulant. However, heparin usage requires an antidote and sensors for safe operation during and after surgeries. In this study, a host–guest complex capable of selective heparin binding and sensing is presented. Heparin binding affinity was studied in solution with a variety of polycationic macrocyclic hosts, a pillar[5]arene and multiple resorcin[4]arenes, by dynamic light scattering, dye displacement assay, isothermal titration calorimetry, and anti-Xa assay. The measurements reveal the significant importance of multivalency in electrostatic host–heparin binding in competitive, application-relevant media. Additionally, to monitor the heparin concentration, a host–guest indicator displacement assay was performed by following the free and bound state of the methyl orange dye in UV-Vis spectroscopic experiments. Furthermore, this colorimetric sensing based on the tertiary host–guest–heparin supramolecular assembly was utilized in the construction of a calibration curve in a range of blood plasma concentrations.

Graphical abstract: A supramolecular host–guest complex for heparin binding and sensing

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Supplementary files

Article information

17 Apr 2018
24 Jun 2018
First published
25 Jun 2018

Nanoscale, 2018,10, 14022-14030
Article type

A supramolecular host–guest complex for heparin binding and sensing

S. Välimäki, N. K. Beyeh, V. Linko, R. H. A. Ras and M. A. Kostiainen, Nanoscale, 2018, 10, 14022
DOI: 10.1039/C8NR03132K

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