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Biocompatible Protamine Sulfate@Silicon Nanoparticles-Based Gene Nanocarriers Featuring Strong and Stable Fluorescence

Abstract

Development of biocompatible and fluorescent gene carriers is of particular importance in the gene-relative field. Taking advantages of unique optical properties (e.g., strong and robust fluorescence) of silicon nanoparticles (SiNPs), as well as excellent biocompatibility of silicon and protamine sulfate (PS, approved by U.S. Food and Drug Administration (FDA) for clinical use), we herein present a kind of PS-modified SiNPs (PS@SiNPs)-based gene carriers. The plasmid DNA (pDNA) with negative charges can be effectively bound onto the surface of the as-prepared fluorescent PS@SiNPs-based gene carriers via electrostatic interactions. In particular, such resultant gene carriers possess stable and high fluorescence (photoluminescent quantum yield (PLQY): ~25% ). In addition, the PS@SiNPs-based gene carriers show minimal toxic effect on normal mitochondrial metabolic activity (e.g., human retinal pigment epithelial (ARPE-19) cells preserve ~90% cell viabilities after 48-h incubation with the resultant carriers). Based on tracking the strong and stable fluorescence signals of SiNPs, the dynamic behaviors of PS@SiNPs-based gene carriers in live cells (e.g., clathrin-mediated endocytosis, lysosomal escape, pDNA release, etc.) are investigated in a long-term manner, providing valuable information for understanding the intracellular behaviors of gene vectors and designing high-efficacy gene carriers.

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Supplementary files

Publication details

The article was received on 17 Apr 2018, accepted on 14 Jun 2018 and first published on 06 Jul 2018


Article type: Paper
DOI: 10.1039/C8NR03107J
Citation: Nanoscale, 2018, Accepted Manuscript
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    Biocompatible Protamine Sulfate@Silicon Nanoparticles-Based Gene Nanocarriers Featuring Strong and Stable Fluorescence

    X. Ji, C. Wang, M. Tang, D. Guo, F. Peng, Y. Zhong, B. Song, Y. Su and Y. He, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR03107J

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