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Immuno-modified superparamagnetic nanoparticles via host-guest interactions for high-purity capture and mild release of exosomes

Abstract

The study on exosomes has great significance for cancers diagnosis and prognosis, in which damage-free isolation of exosomes is a critically important step due to their extremely low concentration in body fluids. Herein, immunoaffinitive superparamagnetic nanoparticles (IS-NPs) were successfully constructed by combining antibodies with superparamagnetic nanoparticles through host-guest interactions between β-cyclodextrin (β-CD) and 4-aminoazobenzene (AAB). Exosomes in cell-culture supernatant or body fluids can be efficiently captured by IS-NPs and then mildly eluted by competitive host molecules α-CD. Approximately 80% exosomes were captured by the IS-NPs from the model samples containing varied concentration of exosomes (1011~107 exosomes/mL). After elution, the release efficiency of captured exosomes was as high as 86.5%, as calculated by extracted RNA content. Comparison between the exosomes isolated by our IS-NPs, conventional ultracentrifugation (UC), polyethylene glycol (PEG)-based precipitation, and commercial Kit was conducted to examine the performance of the IS-NPs exosomes isolation. The particle-to-protein ratio of IS-NPs captured exosomes (8.8±1.3×109) was eight-fold higher than that of the UC (1.1±0.4×109) and more than two-fold of the PEG-based precipitation (3.7±0.8×109) and commercial Kit (3.2±0.8×109) isolated ones, revealing that IS-NPs yielded more exosomes with a higher purity than all the other three approaches. Cellular uptake experiments demonstrated that the exosomes captured by IS-NPs have retained their structural and functional integrities, and their broad applications in biomedical areas could therefore become worth expecting. Promisingly, this host-guest interactions involved immunoaffinity magnetic particle system could offer a new way for fast, high-efficiency and high-purity exosomes isolation and elution, further to benefit exosomes applications in desired areas.

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Publication details

The article was received on 10 Apr 2018, accepted on 11 Jun 2018 and first published on 20 Jun 2018


Article type: Paper
DOI: 10.1039/C8NR02871K
Citation: Nanoscale, 2018, Accepted Manuscript
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    Immuno-modified superparamagnetic nanoparticles via host-guest interactions for high-purity capture and mild release of exosomes

    S. Cai, B. Luo, P. Jiang, X. Zhou, F. Lan, Q. Yi and Y. Wu, Nanoscale, 2018, Accepted Manuscript , DOI: 10.1039/C8NR02871K

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