Jump to main content
Jump to site search

Issue 29, 2018
Previous Article Next Article

Immuno-modified superparamagnetic nanoparticles via host–guest interactions for high-purity capture and mild release of exosomes

Author affiliations

Abstract

The study of exosomes has great significance for cancer diagnosis and prognosis, in which damage-free isolation of exosomes is a critically important step due to their extremely low concentration in body fluids. Herein, immunoaffinitive superparamagnetic nanoparticles (IS-NPs) were successfully constructed by combining antibodies with superparamagnetic nanoparticles through host–guest interactions between β-cyclodextrin (β-CD) and 4-aminoazobenzene (AAB). Exosomes in cell-culture supernatants or body fluids can be efficiently captured by IS-NPs and then mildly eluted by competitive host molecules, α-CD. Approximately 80% of the exosomes were captured by the IS-NPs from the model samples containing varied concentrations of exosomes (1011–107 exosomes per mL). After elution, the release efficiency of the captured exosomes was as high as 86.5%, as calculated from the extracted RNA content. A comparison between the exosomes isolated by our IS-NPs, conventional ultracentrifugation (UC), polyethylene glycol (PEG)-based precipitation, and a commercial kit was conducted to examine the performance of the IS-NP exosome isolation. The particle-to-protein ratio of IS-NP captured exosomes (8.8 ± 1.3 × 109) was eight-fold higher than that of the UC (1.1 ± 0.4 × 109) and more than two-fold that of the PEG-based precipitation (3.7 ± 0.8 × 109) and commercial kit (3.2 ± 0.8 × 109) isolated ones, revealing that IS-NPs yielded more exosomes with higher purity than all the other three approaches. Cellular uptake experiments demonstrated that the exosomes captured by IS-NPs have retained their structural and functional integrities, and their broad applications in biomedical areas could therefore be expected. Promisingly, this host–guest interaction involving immunoaffinity magnetic particle system could offer a new way for fast, high-efficiency and high-purity exosome isolation and elution, further benefitting exosomes’ applications in desired areas.

Graphical abstract: Immuno-modified superparamagnetic nanoparticles via host–guest interactions for high-purity capture and mild release of exosomes

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Apr 2018, accepted on 11 Jun 2018 and first published on 20 Jun 2018


Article type: Paper
DOI: 10.1039/C8NR02871K
Citation: Nanoscale, 2018,10, 14280-14289
  •   Request permissions

    Immuno-modified superparamagnetic nanoparticles via host–guest interactions for high-purity capture and mild release of exosomes

    S. Cai, B. Luo, P. Jiang, X. Zhou, F. Lan, Q. Yi and Y. Wu, Nanoscale, 2018, 10, 14280
    DOI: 10.1039/C8NR02871K

Search articles by author

Spotlight

Advertisements