Issue 3, 2018

Technical challenges of working with extracellular vesicles


Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Graphical abstract: Technical challenges of working with extracellular vesicles

Article information

Article type
Review Article
09 Nov 2017
08 Dec 2017
First published
13 Dec 2017
This article is Open Access
Creative Commons BY license

Nanoscale, 2018,10, 881-906

Technical challenges of working with extracellular vesicles

M. I. Ramirez, M. G. Amorim, C. Gadelha, I. Milic, J. A. Welsh, V. M. Freitas, M. Nawaz, N. Akbar, Y. Couch, L. Makin, F. Cooke, A. L. Vettore, P. X. Batista, R. Freezor, J. A. Pezuk, L. Rosa-Fernandes, A. C. O. Carreira, A. Devitt, L. Jacobs, I. T. Silva, G. Coakley, D. N. Nunes, D. Carter, G. Palmisano and E. Dias-Neto, Nanoscale, 2018, 10, 881 DOI: 10.1039/C7NR08360B

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