Jump to main content
Jump to site search

Issue 11, 2018
Previous Article Next Article

Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

Author affiliations

Abstract

Covering: up to May 2018

Non-ribosomal peptide synthetases (NRPSs) are mega-enzymes that form modular templates to assemble specific peptide products, independent of the ribosome. The autonomous nature of the modules in the template offers prospects for re-engineering NRPS enzymes to generate modified peptide products. Although this has clearly been a primary mechanism of natural product diversification throughout evolution, equivalent strategies have proven challenging to implement in the laboratory. In this review we examine key examples of successful and less-successful re-engineering of NRPS templates to generate novel peptides, with the aim of extracting practical guidelines to inform future efforts. We emphasise the importance of maintaining effective protein–protein interactions in recombinant NRPS templates, and identify strengths and limitations of diverse strategies for achieving different engineering outcomes.

Graphical abstract: Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

Back to tab navigation

Publication details

The article was received on 19 Apr 2018 and first published on 02 Aug 2018


Article type: Review Article
DOI: 10.1039/C8NP00036K
Citation: Nat. Prod. Rep., 2018,35, 1210-1228
  •   Request permissions

    Structural, functional and evolutionary perspectives on effective re-engineering of non-ribosomal peptide synthetase assembly lines

    A. S. Brown, M. J. Calcott, J. G. Owen and D. F. Ackerley, Nat. Prod. Rep., 2018, 35, 1210
    DOI: 10.1039/C8NP00036K

Search articles by author

Spotlight

Advertisements