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A novel fluorescence immunosensor based on Förster resonance energy transfer between nitrogen and sulfur co-doped carbon dots functionalized silica nanospheres and Au@Ag NPs

Abstract

A novel fluorescence (FL) immunosensor for prostate-specific antigen (PSA) detection was developed based on the Förster resonance energy transfer (FRET) between nitrogen and sulfur co-doped carbon dots functionalized silica nanospheres (Si/NS-CDs) and Au@Ag NPs. The strategy was consisted of the PSA primary antibody labeled luminescent Si/NS-CDs as donors and PSA secondary antibody labeled Au@Ag NPs as acceptors. In the existence of PSA, the bio-affinity of the antigen and antibody produced the distance between Si/NS-CDs and Au@Ag NPs close enough, causing the decline of the FL intensity of Si/NS-CDs owing to the FRET effect. By virtue of the Si/NS-CDs nanoprobe with excellent optical properties, the quantitative analysis of PSA has been successfully realized using both FL spectroscopy and a naked-eye readout. The fluorometric results present desirable analytical performance for PSA detection ranged from 5.0 pg mL-1 to 10 ng mL-1 with the detection limit of 0.95 pg mL-1 (S/N = 3). Moreover, satisfactory results from determining PSA target in the samples of human serum were obtained, showing great promising of the method for application in biochemical analysis.

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Publication details

The article was received on 22 Oct 2018, accepted on 03 Dec 2018 and first published on 08 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ05349A
Citation: New J. Chem., 2018, Accepted Manuscript
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    A novel fluorescence immunosensor based on Förster resonance energy transfer between nitrogen and sulfur co-doped carbon dots functionalized silica nanospheres and Au@Ag NPs

    J. Cao, W. Zhang, H. Wang, S. Ma and Y. Liu, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ05349A

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