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A highly sensitive fluorescent probe for selective detection of cyste-ine/homocysteine from glutathione and its application in living cells and tissues

Abstract

Biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play significant roles in maintaining biological homeostasis in the organism. Due to their particular significance in physiology and pathology for human, accurate determination and differentiation of Cys, Hcy, and GSH for understanding their roles in related diseases are in urgent demand. Herein, a new fluorescent probe NIPY-NBD was designed and synthesized for detection of Cys/Hcy and GSH. The probe owned a variety of excellent properties including high sensitivity and low cytotoxicity as well as good cell-membrane permeability. Furthermore, the probe showed a 100-fold (λex/em = 310 nm /520 nm) and 210-fold (λex/em = 340 nm /550 nm) fluorescence enhancement when reacted with Cys in a buffer, and could swiftly respond to Cys and Hcy within 2.5 min. NIPY-NBD was further applied successfully in imaging in A549 cells and xenograft mouse tumor tissues. Taken together, NIPY-NBD may own great potential in detecting and distinguishing Cys /GSH in living animals and could be applied in tumor diagnosis.

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Publication details

The article was received on 16 Aug 2018, accepted on 24 Sep 2018 and first published on 08 Oct 2018


Article type: Paper
DOI: 10.1039/C8NJ04183K
Citation: New J. Chem., 2018, Accepted Manuscript
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    A highly sensitive fluorescent probe for selective detection of cyste-ine/homocysteine from glutathione and its application in living cells and tissues

    Z. Zhou, G. Duan, Y. Wang, S. Yang, X. Liu, L. Zhang, R. Sun, Y. Xu, Y. Gu and X. Zha, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ04183K

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