A novel near-infrared fluorescent probe with a “donor–π–acceptor” type structure and its application in the selective detection of cysteine in living cells

Abstract

The development of imaging-suitable fluorescent probes based on fluorogen scaffolds with near-infrared emission is crucial for monitoring cysteine (Cys) in biological systems. Herein, we synthesized a novel NIR dye based on a “donor–π–acceptor” structure which exhibits good Stokes shifts, satisfactory quantum yields, long wavelength excitation and excellent photostability, and a Cys-responsive NIR probe was constructed by decoration with methacrylate. The designed probe possesses great sensitivity and selectivity for detection of Cys. Moreover, it has been successfully applied in detecting Cys in living cells, indicating its great potential value for biological applications.