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Acetylcholinesterase biosensor modified with ATO/OMC for detecting organophosphorus pesticides

Abstract

This work demonstrated the sensitive amperometric determination of organophosphorus pesticides (OPs) on screen-printed electrode (SPE) modified with antimony tin oxide-chitosan (ATO-CS) and ordered mesoporous carbon-chitosan (OMC-CS) composited nano-material. ATO could effectively promote electron transfer and enhance electrochemical reaction. OMC had a larger specific surface area, so it could effectively fix more AChE and improve the sensitivity of the sensor. Thus, ATO-CS and OMC-CS were modified onto the SPE layer by layer, which could improve the electrochemical response, increase the fixed amount of the enzyme on the electrode, and accordingly the sensitivity of the biosensor was obviously improved. The electrochemical behavior of the AChE biosensor was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) technique. Under optimum conditions, with chlorpyrifos and methamidophos as model compounds, inhibition of AChE activity based on OPs was detected. The proposed acetylcholinesterase biosensor displayed an excellent analytical performance with great reproducibility. And the detection limits for chlorpyrifos and methamidophos were 0.01 μg/L and 1 μg/L respectively. In addition, the as-prepared acetylcholinesterase biosensor was successfully utilized for the determination of OPs in actual sample.

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Publication details

The article was received on 10 Jul 2018, accepted on 17 Oct 2018 and first published on 19 Oct 2018


Article type: Paper
DOI: 10.1039/C8NJ03429J
Citation: New J. Chem., 2018, Accepted Manuscript
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    Acetylcholinesterase biosensor modified with ATO/OMC for detecting organophosphorus pesticides

    Q. Zhang, W. Hou, F. Li, Y. Zhang, Y. Guo and X. Sun, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ03429J

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