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Issue 20, 2018
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A label-free ratiometric fluorescence strategy for 3′–5′ exonuclease detection

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Abstract

A label-free ratiometric fluorescent platform has been developed for the detection of 3′–5′ exonuclease utilizing two individual DNA conformation-specific dyes. Initially, the DNA substrate maintains a stable HP (hairpin probe) structure, resulting in bright fluorescence at 455 nm for 4′,6-diamidino-2-phenylindole (DAPI), which is able to insert into A–T base pairs of the HP structure. Upon the addition of 3′–5′ exonuclease (exonuclease III), the HP structure was digested to single-stranded DNA (ssDNA), and the fluorescence intensity at 455 nm was decreased. However the emission of N-methyl mesoporphyrin IX (NMM) at 610 nm was increased significantly due to the formation of G-quadruplex. This label-free method is realized by facile design and shows good selectivity for Exo III detection. More importantly, it also shows its potential as a platform for Exo III inhibitor screening.

Graphical abstract: A label-free ratiometric fluorescence strategy for 3′–5′ exonuclease detection

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Publication details

The article was received on 29 Jun 2018, accepted on 31 Aug 2018 and first published on 01 Sep 2018


Article type: Paper
DOI: 10.1039/C8NJ03242D
Citation: New J. Chem., 2018,42, 16630-16634
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    A label-free ratiometric fluorescence strategy for 3′–5′ exonuclease detection

    W. Hu, H. Zhao, J. Jing and X. Zhang, New J. Chem., 2018, 42, 16630
    DOI: 10.1039/C8NJ03242D

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