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Enantioselective open-tubular capillary electrochromatography using β-cyclodextrin-gold nanoparticles-polydopamine coating as stationary phase

Abstract

A novel enantioselective open-tubular capillary electrochromatography (OT-CEC) was developed employing thiols β-cyclodextrin (SH-β-CD) conjugated polydopamine-gold nanoparticles (PDA-AuNPs) as stationary phase. The capillary inner surface was functionalized with dopamine by self-polymerization under alkaline condition and subsequent introduction of gold nanoparticles based on the electrostatic and affinity interaction between gold nanoparticles and amine groups on polydopamine. Then, thiols β-cyclodextrin was introduced on the gold nanoparticles layer through S–Au covalent bonding. The preparation conditions including dopamine concentration, the numbers of coating layer of gold nanoparticles and thiols β-cyclodextrin concentration were optimized to obtain satisfactory enantioselectivity. Scanning electron microscopy (SEM), energy dispersive X-ray analysis spectroscopy (EDS) and EOF experiments were carried out to characterize the prepared open tubular (OT) column. Then, the separation effectiveness of the open tubular column was verified by the separation of seven chiral drugs including tryptophan, phenylalanine, histidine, fexofenadine, promethazine, tropicamide and terbutaline, and satisfactory separation results were obtained for the analytes studied. Moreover, the stability and repeatability of the column were studied through the intra-day, inter-day and column-to-column investigations.

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Publication details

The article was received on 30 Jun 2018, accepted on 10 Sep 2018 and first published on 11 Sep 2018


Article type: Paper
DOI: 10.1039/C8NJ03234C
Citation: New J. Chem., 2018, Accepted Manuscript
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    Enantioselective open-tubular capillary electrochromatography using β-cyclodextrin-gold nanoparticles-polydopamine coating as stationary phase

    L. Zhou, B. Zhang, S. Li, J. Yu and X. Guo, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ03234C

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