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Issue 21, 2018
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The capsid assembly-induced luminescence enhancement (AILE) of DNA-protected silver nanoclusters and an in situ application

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Abstract

Two assembly modes have been constructed between double-strand DNA-protected silver nanoclusters (AgNCs-dsDNA) and human papillomavirus (HPV) 16 main capsid protein L1, respectively, which are well defined by CsCl gradient centrifugation, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and transmission electron microscopy (TEM). The co-assembly leads to a large emission increase of AgNCs-dsDNA attributed to an assembly-induced luminescence enhancement (AILE), and vice versa in disassembly; however, the post-assembly does not. Therefore, a new approach to monitoring the assembly/disassembly of HPV virus-like particles (VLPs) is established accordingly. The study not only presents an AILE phenomenon for silver nanoclusters but also affords an easily performed fluorescence approach to evaluate HPV VLP assembly/disassembly directly, which could be extended to other kinds of metal nanoclusters protected by DNA as well as a kinetic study on the assembly of other species of virus.

Graphical abstract: The capsid assembly-induced luminescence enhancement (AILE) of DNA-protected silver nanoclusters and an in situ application

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Publication details

The article was received on 27 Jun 2018, accepted on 14 Sep 2018 and first published on 15 Sep 2018


Article type: Paper
DOI: 10.1039/C8NJ03179G
Citation: New J. Chem., 2018,42, 17492-17498
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    The capsid assembly-induced luminescence enhancement (AILE) of DNA-protected silver nanoclusters and an in situ application

    X. Jia, Y. Xue, H. Li, D. Fu, W. Wang and Y. Wu, New J. Chem., 2018, 42, 17492
    DOI: 10.1039/C8NJ03179G

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