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The Capsid Assembly-induced Luminescence Enhancement (AILE) of DNA-protected Silver Nanoclusters and an in-situ Application

Abstract

Two assembly modes have been constructed between double-strand DNA-protected silver nanoclusters (AgNCs-dsDNA) and human papillomavirus (HPV) 16 main capsid protein L1, respectively, which are well defined by CsCl gradient centrifugation, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and transmission electron microscopy (TEM). The co-assembly leads to large emission increase of AgNCs-dsDNA being attributed to assembly-induced luminescence enhancement (AILE), and vice versa in disassembly; however, the post-assembly does not. Therefore, a new approach to monitoring the assembly/disassembly of HPV virus-like particles (VLPs) is established accordingly. The study not only presents an AILE phenomenon for silver nanoclusters but also affords an easily performed fluorescence approach to evaluate the HPV VLPs assembly/disassembly directly, which would be extended to other kinds of metal nanoclusters protected by DNA as well as kinetic study on other species of virus assembly.

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Publication details

The article was received on 27 Jun 2018, accepted on 14 Sep 2018 and first published on 15 Sep 2018


Article type: Paper
DOI: 10.1039/C8NJ03179G
Citation: New J. Chem., 2018, Accepted Manuscript
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    The Capsid Assembly-induced Luminescence Enhancement (AILE) of DNA-protected Silver Nanoclusters and an in-situ Application

    X. Jia, Y. Xue, H. Li, D. Fu, W. Wang and Y. Wu, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ03179G

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