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Imaging immunoassay in negative: surface-catalysed chemiluminescence for the detection of pregnancy hormones in artificial saliva

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Abstract

A novel and rapid (45 min), quantitative chemiluminescence-based, surface immunoassay is reported for the detection of progesterone and œstriol in artificial saliva. The detection limits for these pregnancy hormones are 2.3 and 2.5 pg mL−1, respectively. The assay is based on the use of ferrocene-tagged, monoclonal antibodies immobilised on a surface, so that the oxidised ferricenium catalyses the reaction between luminol and hydrogen peroxide. The immunoassay is performed in negative, such that increasing the antigen concentration gives rise to decreasing light intensity that is observed, and is unaffected by antibody orientation on the surface. This affords a method of calibration that is readily translated to pregnancy hormone detection in a primary point-of-care environment. Biomolecules with similar structures to these pregnancy hormones found in saliva are demonstrated not to interfere with the immunoassay.

Graphical abstract: Imaging immunoassay in negative: surface-catalysed chemiluminescence for the detection of pregnancy hormones in artificial saliva

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Publication details

The article was received on 14 Jun 2018, accepted on 12 Oct 2018 and first published on 12 Oct 2018


Article type: Paper
DOI: 10.1039/C8NJ02974A
Citation: New J. Chem., 2018, Advance Article
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    Imaging immunoassay in negative: surface-catalysed chemiluminescence for the detection of pregnancy hormones in artificial saliva

    K. J. Wright, Z. O. Oiaidha, D. P. Love, M. Aljohani, G. M. Greenway and J. D. Wadhawan, New J. Chem., 2018, Advance Article , DOI: 10.1039/C8NJ02974A

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