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A novel fluorescence strategy for mercury ions and trypsin activity assay based on nitrogen-doped graphene quantum dots

Abstract

In this paper, we developed a simple, convenient and sensitive fluorescent probe for mercury ions (Hg2+) and trypsin activity assay based on nitrogen-doped graphene quantum dots (N-GQDs). Hg2+ can quench the fluorescence of N-GQDs due to the electron transfer from N-GQDs to Hg2+. However, amino acids or peptide fragments formed by hydrolysis of human serum albumin (HSA) in the presence of trypsin have stronger affinity for Hg2+, thus the fluorescence of N-GQDs will be recovered after the addition of HSA and trypsin. Hg2+ and trypsin activity could be monitored via the change of fluorescent intensity of N-GQDs. Under optimal conditions, the fluorescence intensity of N-GQDs was proportional to the concentration of Hg2+ and trypsin in the range of 0.02-1 μM and 0.03-8 μg/mL with a limit of 0.0047 μM and 0.0063 μg/mL, respectively. Besides, soybean trypsin inhibitor (SBTI) as a model for trypsin inhibition was used to confirm the feasibility of the system and the value of IC50 is 1.31 μg/mL. The proposed method was successfully applied to the determination of Hg2+ and trypsin activity in real samples with satisfactory results.

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Publication details

The article was received on 05 Jun 2018, accepted on 03 Sep 2018 and first published on 07 Sep 2018


Article type: Paper
DOI: 10.1039/C8NJ02790K
Citation: New J. Chem., 2018, Accepted Manuscript
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    A novel fluorescence strategy for mercury ions and trypsin activity assay based on nitrogen-doped graphene quantum dots

    D. Su, M. Wang, Q. Liu, Z. Qu and X. Su, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02790K

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