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Issue 15, 2019
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SERS based protocol using flow glass-hemostix for detection of neuron-specific enolase in blood plasma

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Abstract

An inexpensive and disposable lateral flow glass-hemostix (FGH) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. Gold nanocage@Raman reporter nanoparticles are developed as SERS tags, which is the key to the high sensitivity of the device. Compared with ELISA, the SERS-FGH exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-FGH has been successfully used for the detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.74 ng mL−1. Moreover, the SERS-FGH was successfully employed to measure the NSE level in clinical blood plasma samples taken from identified TBI patients. This work demonstrates that the SERS-FGH has great potential in assisting screening of TBI patients in the point-of-care setting.

Graphical abstract: SERS based protocol using flow glass-hemostix for detection of neuron-specific enolase in blood plasma

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Publication details

The article was received on 24 May 2018, accepted on 15 Jul 2018 and first published on 21 Aug 2018


Article type: Paper
DOI: 10.1039/C8NJ02561D
Citation: New J. Chem., 2019,43, 5925-5931

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    SERS based protocol using flow glass-hemostix for detection of neuron-specific enolase in blood plasma

    D. Li, M. Yang, H. Li, L. Mao, Y. Wang and B. Sun, New J. Chem., 2019, 43, 5925
    DOI: 10.1039/C8NJ02561D

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