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SERS Based protocol Flow Glass-hemostix for Detection of Neuron-Specific Enolase in Blood Plasma

Abstract

An inexpensive and disposable based lateral flow glass-hemostix (FGH) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. The gold nanocage@Raman reporter nanoparticles are developed as the SERS tags, which is the key to the high sensitivity of the device. Compared with ELISA the SERS-FGH exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-FGH has been successfully used for detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.74 ng/mL. Moreover, the SERS-FGH was successfully employed to measure the NSE level in clinical blood plasma samples taken from deidentified TBI patients. This work demonstrates that the SERS-FGH has great potential in assisting screening of TBI patients in the point of care setting.

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Publication details

The article was received on 24 May 2018, accepted on 15 Jul 2018 and first published on 21 Aug 2018


Article type: Paper
DOI: 10.1039/C8NJ02561D
Citation: New J. Chem., 2018, Accepted Manuscript
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    SERS Based protocol Flow Glass-hemostix for Detection of Neuron-Specific Enolase in Blood Plasma

    D. Li, M. Yang, H. Li, L. Mao, Y. Wang and B. Sun, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02561D

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