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A printable voltammetric genosensor for a tumour suppressor gene screening based on nanocomposite of Ceria NPs-GO/nano-PANI

Abstract

A novel voltammetric screen printed genosensor has been developed for detecting mutation in a particular sequence of adenomatous polyposis coli (APC) gene which is classified as a tumor suppressor gene. This gene prevents uncontrolled growth of cells which can cause cancerous tumors. For this purpose, a screen-printed carbon electrode (SPCE) surface was modified with nano-polyaniline (nano-PANI) and graphene oxide (GO)-ceria nanocomposite (Ceria NPs-GO/nano-PANI/ SPCE). Ceria NPs-GO was a suitable base for label-free immobilizaition of a single strand oligonucleotide probe. The composite was able to catalyze a difference pulse voltammetry (DPV) redox signal of Tris(bipyridine) ruthenium(II) chloride utilized as a redox probe for monitoring the hybridization of DNA. The genosensor displayed excellent performance over the linear range from 1×10--5 to 5×10-2 µmol L-1 with limit of detection, 5.7×10-6 µmol L-1. Also, it can distinguish a target sequence from three mismatch bases sequences successfully.

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Publication details

The article was received on 17 May 2018, accepted on 07 Aug 2018 and first published on 07 Aug 2018


Article type: Paper
DOI: 10.1039/C8NJ02437E
Citation: New J. Chem., 2018, Accepted Manuscript
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    A printable voltammetric genosensor for a tumour suppressor gene screening based on nanocomposite of Ceria NPs-GO/nano-PANI

    M. Eskandari and F. Faridbod, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02437E

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