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A NBD-S-rhodamine dyad for dual-color discriminative imaging of biothiols and Cys/Hcy

Abstract

Biothiols are essential biomolecules in sustaining intracellular redox homeostasis. Abnormal levels of Cys, Hcy, GSH and H2S are linked to different diseases, respectively. Although many fluorescent probes are successfully applied in detection of these biothiols in living biological samples, probes with high selectivity to discriminatively detect the biothiols are underdeveloped. Herein, we designed and synthesized a new dual-color NBD-S-rhodamine fluorescent probe based on fast thiolysis of NBD thioether, a non-thiol-consumption receptor. The probe could react with GSH/H2S to give red fluorescence, and with Cys/Hcy to give both green and red fluorescence for discriminative detection of biothiols and Cys/Hcy. Results revealed that this probe exhibited good selectivity and high sensitivity toward biothiols, and could be used in bioimaging. Based on the probe tool, we could selectively detect Cys/Hcy in the presence of GSH in living mammalian cells.

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Publication details

The article was received on 11 May 2018, accepted on 03 Aug 2018 and first published on 06 Aug 2018


Article type: Paper
DOI: 10.1039/C8NJ02323A
Citation: New J. Chem., 2018, Accepted Manuscript
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    A NBD-S-rhodamine dyad for dual-color discriminative imaging of biothiols and Cys/Hcy

    L. Sun, Y. Jiang, C. Zhang, X. Ji, D. Lv, Z. Xi and L. Yi, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02323A

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