Jump to main content
Jump to site search


A smart supramolecular device for the detection of t,t-muconic acid in urine

Abstract

In this work, we combined smartphone sensing with the Indicator Displacement approach for the determination of trans,trans-muconic acid (i.e. tt-MA), a benzene biomarker, in urine. As receptor, a dicopper(II) azacryptate was employed. The good match of tt-MA with the receptor cavity was suggested by DFT calculations, and confirmed experimentally by UV-vis. titrations in buffered aqueous solution. The binding constant was determined through fluorimetric titrations, using the Indicator Displacement approach. This method also confirmed the high affinity of the azacryptate for tt-MA in artificial and spiked urines. A portable device was then obtained by adsorbing the chemosensing ensemble on silica gel, in ELISA-like plastic wells as support. Indicator Displacement was detected and quantifyed by exposing the microplate to a UV-lamp (366 nm) and recording the RGB values with a Smartphone. A good correlation was found between the R index values and urinary tt-MA in the occupational concentration range.

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 May 2018, accepted on 06 Aug 2018 and first published on 10 Aug 2018


Article type: Paper
DOI: 10.1039/C8NJ02156B
Citation: New J. Chem., 2018, Accepted Manuscript
  •   Request permissions

    A smart supramolecular device for the detection of t,t-muconic acid in urine

    D. Merli, S. La Cognata, F. Balduzzi, A. Miljkovic, L. Toma and V. Amendola, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02156B

Search articles by author

Spotlight

Advertisements