A sensitive two-photon ratiometric fluorescent probe for γ-glutamyltranspeptidase activity detection and imaging in living cells and cancer tissues†
Abstract
We develop a novel two-photon ratiometric fluorescent probe based on the conjugation of the specific recognition moiety of the γ-glutamyl group to 6-acetyl-2-aminonaphthalene, which enables highly sensitive, selective monitoring of γ-glutamyltransferase activities in human serum and imaging in living cells and cancer tissues down to a depth of 120 μm.