Issue 6, 2018

Engineering hyperthermostable rcSso7d as reporter molecule for in vitro diagnostic tests

Abstract

The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conjugation methods to link a detectable signal to the presence of a target biomarker.

Graphical abstract: Engineering hyperthermostable rcSso7d as reporter molecule for in vitro diagnostic tests

Supplementary files

Article information

Article type
Communication
Submitted
31 Jul 2018
Accepted
15 Oct 2018
First published
15 Oct 2018
This article is Open Access
Creative Commons BY-NC license

Mol. Syst. Des. Eng., 2018,3, 877-882

Engineering hyperthermostable rcSso7d as reporter molecule for in vitro diagnostic tests

K. Sung, E. A. Miller and H. D. Sikes, Mol. Syst. Des. Eng., 2018, 3, 877 DOI: 10.1039/C8ME00049B

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