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Issue 23, 2018
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Integration of sample preparation and analysis into an optofluidic chip for multi-target disease detection

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Abstract

Detection of molecular biomarkers with high specificity and sensitivity from biological samples requires both sophisticated sample preparation and subsequent analysis. These tasks are often carried out on separate platforms which increases required sample volumes and the risk of errors, sample loss, and contamination. Here, we present an optofluidic platform which combines an optical detection section with single nucleic acid strand sensitivity, and a sample processing unit capable of on-chip, specific extraction and labeling of nucleic acid and protein targets in complex biological matrices. First, on-chip labeling and detection of individual lambda DNA molecules down to concentrations of 8 fM is demonstrated. Subsequently, we demonstrate the simultaneous capture, fluorescence tagging and detection of both Zika specific nucleic acid and NS-1 protein targets in both buffer and human serum. We show that the dual DNA and protein assay allows for successful differentiation and diagnosis of Zika against cross-reacting species like dengue.

Graphical abstract: Integration of sample preparation and analysis into an optofluidic chip for multi-target disease detection

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Publication details

The article was received on 11 Sep 2018, accepted on 18 Oct 2018 and first published on 20 Oct 2018


Article type: Paper
DOI: 10.1039/C8LC00966J
Citation: Lab Chip, 2018,18, 3678-3686
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    Integration of sample preparation and analysis into an optofluidic chip for multi-target disease detection

    G. G. Meena, A. Jain, J. W. Parks, A. Stambaugh, J. L. Patterson, A. R. Hawkins and H. Schmidt, Lab Chip, 2018, 18, 3678
    DOI: 10.1039/C8LC00966J

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