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Enhanced parylene-C fluorescence as a visual marker for neuronal electrophysiology applications

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Abstract

Parylene-C is a popular polymer material in biomedical applications, with excellent physicochemical properties and microfabrication capability. Like many aromatic polymers, parylene-C also has autofluorescence, which was usually taken as a negative background noise in biomedical detection studies. However, the fluorescence intensity of thin-film (<1 μm) parylene-C was relatively weak, which may be a big limitation in visualization. In this work, we reported a simple annealing method to significantly enhance the fluorescence and achieve sufficient intensity as a visual marker. We studied the behaviors and mechanisms of the enhanced parylene-C fluorescence, then verified the feasibility and reliability of parylene-C for preparing fluorescent pipettes in targeted neuronal electrophysiology, where fluorescent guidance was strongly needed. The powerful parylene-C fabrication technique enables a precisely-controlled conformal coating along with a mass production capability, which further resulted in high-quality electrophysiological recordings of both cultured hippocampal neurons and acute hippocampal brain slices. Moreover, the enhanced parylene-C fluorescence can also be applied in more general biological operations, such as designable fluorescent micro-patterns for visualization in broader biomedical fields.

Graphical abstract: Enhanced parylene-C fluorescence as a visual marker for neuronal electrophysiology applications

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Publication details

The article was received on 03 Aug 2018, accepted on 01 Nov 2018 and first published on 02 Nov 2018


Article type: Communication
DOI: 10.1039/C8LC00804C
Citation: Lab Chip, 2018, Advance Article
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    Enhanced parylene-C fluorescence as a visual marker for neuronal electrophysiology applications

    L. Zhang, M. Wei, L. Shao, M. Li, W. Dai, Y. Cui, Z. Li, C. Zhang and W. Wang, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00804C

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