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Issue 22, 2018
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Self-filling microwell arrays (SFMAs) for tumor spheroid formation

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Abstract

Tumor spheroid formation in microwell arrays is a promising approach for high-throughput screening of chemotherapeutic agents. This method offers the advantage of better mimicking the complexities of tumors as compared to conventional monolayer culture systems. However, using these technologies to their full potential is hindered by the inability to seed the cells within the wells uniformly and with high yield and reproducibility. Moreover, standard manufacturing approaches for fabrication of microwell arrays rely on lithography and etching techniques, which are costly, labor-intensive, and time-consuming. Herein, we report on the development of self-filling microwell arrays (SFMAs) in which cells are directed from a loading chamber to microwells using inclined guiding channels. The SFMAs are fabricated by replica molding of three-dimensionally (3D) printed molds in agarose. We characterize the fabrication process, demonstrate the ability to culture breast adenocarcinoma MCF-7 and glioma U87 in SFMAs and perform drug toxicity studies. We envision that the proposed innovative approach opens avenues of opportunities for high-throughput three-dimensional cell culture for drug screening and disease modeling.

Graphical abstract: Self-filling microwell arrays (SFMAs) for tumor spheroid formation

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Supplementary files

Article information


Submitted
06 Jul 2018
Accepted
06 Oct 2018
First published
08 Oct 2018

Lab Chip, 2018,18, 3516-3528
Article type
Paper

Self-filling microwell arrays (SFMAs) for tumor spheroid formation

A. Seyfoori, E. Samiei, N. Jalili, B. Godau, M. Rahmanian, L. Farahmand, K. Majidzadeh-A and M. Akbari, Lab Chip, 2018, 18, 3516
DOI: 10.1039/C8LC00708J

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