Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 21, 2018
Previous Article Next Article

Integrated measurement of intracellular proteins and transcripts in single cells

Author affiliations

Abstract

Biological function arises from the interplay of proteins, transcripts, and metabolites. An ongoing revolution in miniaturization technologies has created tools to analyze any one of these species in single cells, thus resolving the heterogeneity of tissues previously invisible to bulk measurements. An emerging frontier is single cell multi-omics, which is the measurement of multiple classes of analytes from single cells. Here, we combine bead-based transcriptomics with microchip-based proteomics to measure intracellular proteins and transcripts from single cells and defined small numbers of cells. The transcripts and proteins are independently measured by sequencing and fluorescent immunoassays respectively, to preserve their optimal measurement modes, and linked by encoding the physical address locations of the cells into digital sequencing space using spatially patterned DNA barcodes. We resolve cell-type-specific protein and transcript signatures and present a path forward to scaling the platform to high-throughput.

Graphical abstract: Integrated measurement of intracellular proteins and transcripts in single cells

Back to tab navigation

Supplementary files

Article information


Submitted
22 Jun 2018
Accepted
24 Aug 2018
First published
27 Aug 2018

Lab Chip, 2018,18, 3251-3262
Article type
Paper

Integrated measurement of intracellular proteins and transcripts in single cells

A. M. Xu, Q. Liu, K. L. Takata, S. Jeoung, Y. Su, I. Antoshechkin, S. Chen, M. Thomson and J. R. Heath, Lab Chip, 2018, 18, 3251
DOI: 10.1039/C8LC00639C

Social activity

Search articles by author

Spotlight

Advertisements