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Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration

Abstract

We report the design and characterization of a lateral and vertical hydrodynamic focusing feature for whole cell detection on a miniaturized flow cytometer. The developed system, based on magnetic sensing, incorporates Spin Valve sensors on the bottom of the microfluidic channels that detect cells labelled with magnetic beads. An adaptable 3D hydrodynamic focusing system was developed that pushes labelled cells towards the bottom of the microchannel, closer to the sensors, allowing increased signal for cells labelled with magnetic beads and enhanced discrimination of labelled cells. Fluorescence microscopy indicate that the lateral and vertical hydrodynamic focusing effect was adequately implemented, consistent with simulation predictions. The sensitivity of the system to detect labelled cells was improved by at least two-fold. By estimating the coverage of magnetic beads on cells, the signal from labelled cells could be predicted using a mathematical model, which also demonstrated the sensitivity of the signal to the height of the cells relative to the sensor. The system is versatile allowing interchangeable flow rates for cells with different diameters.

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Publication details

The article was received on 11 May 2018, accepted on 05 Jul 2018 and first published on 07 Jul 2018


Article type: Paper
DOI: 10.1039/C8LC00486B
Citation: Lab Chip, 2018, Accepted Manuscript
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    Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration

    A. Chicharo, M. Martins, L. C. Barnsley, A. Taouallah , J. Fernandes, B. F. B. Silva, S. Cardoso, L. Diéguez, E. Begoña and P. P. Freitas, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C8LC00486B

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